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Insulin-like Growth Factors (IGF´s), Biotinylated IGF´s and Analogs, Biotinylated Insulin
Rat IGF-II, recombinant protein (Receptor Grade) (Kopie 1)
Insulin-like Growth Factor-II, Rat, Receptor Grade, recombinant Synonyms: insulin like growth factor-2, IGF-2, Somatomedin A, ILGF2, T3M-11-derived growth factor,
Product Specifications Rat IGF-II:
Rat Insulin-like Growth Factor-II is polypeptide of 67 amino acids, four of which differ from human IGF-II at positions 22, 32, 35 and 36, with the latter three changes occuring in the C-domain.
Reference:Dull TJ et al (1986) Nature 310, 771-781.
Source: Produced recombinantly in E. coli
Purity: > 95% (as determined by HPLC and N-terninal sequence analysis
Molecular weight: 7515 Da, confirmed by Mass Spectrometry
Appearance:Lyophilized white powder
N-terminal sequence analysis: 5 residues > 95% single sequence
Endotoxin (LAL): < 0.01 EU/µg
Biological Activity: Rat IGF-II has a similar potency to human IGF-II for binding to the Type 2 IGF receptors on rat L6 myoblasts. Both ED50 values are approximately 3.8 ng/ml. Rat IGF-II and human IGF-II also exhibit a similar potency for the stimulation of protein synthesis in rat L6 myoblasts. In this assay both ED50 values are approximately 15 ng/ml.
State:Dried from 0.1 M acetic acid and stored under dry nitrogen at a slight vacuum.
Reconstitution:Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilution (e.g. < 1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles.
Storage/Stability: At least 2 years at 2-4°C (lyophilized)
This is a Gropep specification. ibt is authorized distributor of GroPep.
Rev. 2013-02
Datasheet for Rat IGF-II, recombinant protein (Receptor Grade) (Kopie 1)