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Human IGFBP-4, recombinant protein (Receptor Grade)
Insulin-like Growth Factor Binding Protein-4, human, recombinant Synonyms: Igf binding protein 4; Ibp4, HT29-IGFBP (HT29 insulin-like growth factor binding protein),
Product Specifications:
Human Insulin-like Growth Factor Binding Protein-4 (rhIGFBP-4) is a 237 amino acid glycosylated protein that is one of the six 'classical' IGF binding proteins. These binding proteins have an important role in stabilizing and regulating the activity of IGFs in vivo. The product, purified by IGF affinity chromatography, contains dimers of hIGFBP4 by SDS-PAGE and also <10% CHO cell derived native hamster IGFBP-4.
References:
Neumann GM et al (1998) Biochemistry 37, 6572-6585.
Shimasaki, S et al (1990) Molecular Endocrinology 4, 1451-1458
Source: Produced recombinantly in mammalian cells. Purified
by IGF affinity and other chromatographic steps.
Purity: > 90% (as determined by N-terminal sequencing)
Molecular weight: 24 - 28 kDa (glycosylated). Confirmed by SDS -
PAGE under reducing conditions.
Quantity: 20 µg
Appearance: Lyophilized white powder
Biological Activity: Western Ligand Blot with biotinylated IGF-I and IGF-II
State: Dried from 100 mM acetic acid
Reconstitution: Reconstitute in 100 µl of 10 mM HCl at a concentration of 0.2 mg/ml. Aliquot, snap freeze and store at -80°C. Avoid freeze - thaw cycles.
Storage/Stability: At least 2 years at 2-4°C (lyophilized)
This a GroPep Specification! ibt is authorized distributor of GroPep!
Rev2013-03
Datasheet for Human IGFBP-4, recombinant protein (Receptor Grade)