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Des(1-6)IGF-II, recombinant protein (Receptor Grade)
Des(1-6) Insulin-like Growth Factor-II, recombinant Synonyms: Des(1-6)IGF-2, Des(1-6) Insulin-like Growth Factor-2
Produt Specifications Des(1-6)IGF-II:
The Product: Des(1-6) insulin-like growth factor - II [Des(1-6)IGF-II] is a 61 amino acid analog of human IGF-II, lacking the first six N-terminal amino acids. Des(1-6)IGF-II exhibits reduced affinity for IGF binding proteins (IGFBPs), which are known to inhibit the actions of IGF's. Des(1-6)IGF-II has slightly lower affinity for IGFBPs than the IGF-II analog [Arg6]IGF-II
Source: Des(1-6)IGF-II is produced recombinantly in E. coli using a patented expression system and chromatography is employed to yield Receptor Grade Des(1-6)IGF-II.
Purity: >95% (as determined by HPLC and N-terminal sequence analysis)
Molecular Weight: 6765 daltons - confirmed by Mass Spectrometry
N-terminal sequence analysis of recombinant product: 5 residues > 95% single sequence
Biological Activity: (stimulation of protein synthesis in rat L6 myoblasts) ED50 <75 ng/ml
Endotoxin (LAL): <0.1 EU/ug
Stability: At least 2 years at 2-4°C (lyophilised)
Appearance: White Powder
State: Dried from 0.1 M acetic acid: under dry nitrogen @ slight vacuum (-25kPa)
Reconstitution:Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilutions (e.g.< 1mg/ml) inclusion of a carrier-protein is recommended. Avoid freeze-thaw cycles.
This is a GroPep specification. ibt is authorized distributor of GroPep.
Rev. 2013-02
Datasheet for Des(1-6)IGF-II, recombinant protein (Receptor Grade)