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Anti-human TSH, monoclonal, mouse
Product specifications monoclonal mouse anti-human thyroid stimulating hormone (TSH):
Clones: 11E4, 7G12, 10C7, 5E8, TSB1, TSB4.
Description: clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with TSH from human pituitary gland.
Specificity: Clone 11E4 specifically recognizes β-subunit of human TSH. Clones 7G12, 10C7 and 5E8 specifically recognize the whole molecule of TSH. Clones 7G12, 10C7 and 5E8 do not cross-react with human LH, FSH, HCG.
Clones TSB1 and TSB4 specifically recognize β-subunit of human TSH. Clones TSB1 and TSB4 do not cross-react with human LH, FSH, HCG.
Isotype: IgG1 for all Clones.
Applications: Detection of human TSH in two-site ELISA. Recommended pairs (capture - conjugate): 11E4 - 7G12, 10C7 - 5E8, TSB1 - TSB4 (fab fragments).
Purification: Clones 11E4, 7G12, 10C7 and 5E8 by chromatography on protein A Sepharose
Clones TSB1 and TSB4 by ion exchange chromatography
Formulation: Clones 11E4, 7G12, 10C7 and 5E8 in PBS, pH 7.4, < 0.1 % sodium azide (NaN3). Clones TSB1 and TSB4 in 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05% sodium azide (NaN3).
Storage: + 4 °C.
Datasheet for Anti-human TSH, monoclonal, mouse