Product Specifications Monoclonal Mouse Anti-human Insulin C-peptide:

Clones: 1H8, 2B7, 4H8, 5B8, 7E10, 2A11.

Isotypes: IgG1, kappa for all Clones.
Description: Clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with recombinant C-peptide.

Specificity: No cross-reactivity with human, bovine, porcine and mouse/rat insulin.
Affinity: (Kd) > 1 x 108 M for all Clones.
Applications: All Clones (except 2B7, 7E10 and 2A11) bind with equal efficiency to the human free C-peptide and C-peptide region in proinsulin molecules. Antigenic determinants identified by Clones are in the three-dimensional structure generated by N- and C- terminal regions of C-peptide separated by beta-turn at position 47-50 of proinsulin.

Competitive ELISA or RIA detection of C-peptide in human serum after immunospecific removal of proinsulin (with solid-phased 1D6 or 1D3 Clones to human proinsulin).
Sandwich EIA/ RIA immunosorbent assay for the measurement of total human proinsulin (including proinsulin convertion intermediates) in serum.
Specific immunosorbtional removal of total proinsulin and C-peptide molecules from serum samples (with solid-phase 1H8, 2B7 and 4H8) before the measurement of human insulin.

Purification: Chromatography on protein A Sepharose.

Formulation: PBS, pH 7.4,< 0.1 % sodium azide (NaN3).

Storage: + 4 °C.

 Datasheet for Anti-human Insulin C-peptide, monoclonal, mouse