Product Specifications Monoclonal Mouse Anti-Rat Insulin C-Peptide:

Clones: 6H1, CII-11, CII-29, CII-55, CII-97, CII-106, CII-138, CI-0,  CC24, CC27, CC34.
Description: Clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with fragments of rat C-peptides I and II conjugated with the carrier protein.

Specificity:

Isotype: IgG1 for Clones CII-11, CII-29, CII-55, CII-97, CII-106, CII-138, 6H1, CI-0, CC18, CC24, CC27, CC34.

Applications: All Clones recognize rat C-peptide in ELISA and Sandwich type immunoassay.
Clones can be used for separate or consistent detection of rat C-peptide isoforms (rat C-peptide I and/or rat C-peptide II) in biological samples.

Best pairs for sandwich immunoassays. For sandwich immunoassay the general recommendation is to use combinations of MAbs named as “CC” (MAbs specific to C-terminal part of rat C-peptide) and MAbs named as ”CI” or “CII” (MAbs specific to N-terminal part of rat C-peptide).

The most sensitive pairs recommended for sandwich-type rat C-peptide immunoassays are (capture-detection):

Rat C-peptides I and II immunodetection:

CII-29 – CC24, CII-55 – CC24, CII-97 – CC24, C34 – CII-11, CC27 – CII-29.

Rat C-peptide I immunodetection:

CC34 – 6H1, CC34 – CI-0, 6H1 – CC24.

Rat C-peptide II immunodetection:

CC18 – CII-106, CC34 – CII-106, CC34 – CII-138.

Purification: Chromatography on protein A Sepharose

Formulation: PBS, pH 7.4, < 0.1 % sodium azide (NaN3)

Storage:+ 4 °C.

 Datasheet for Anti-rat Insulin C-peptide, monoclonal, mouse