Anti-human IGF-II Polyclonal Antiserum Specifications

Product Code: PAC1

   The Product: Anti-human IGF-II is a polyclonal antiserum specific
   for human, rat, chicken and porcine IGF-II amongst the species tested.
   Cross reactivity with either ovine, insulin, human, rat or chicken
   IGF-I is undetectable. This polyclonal antiserum is suitable for use
   in radioimmunoassay, immunoblotting and immunoprecipitation.

   Source: The antiserum is derived from rabbit serum raised
   against recombinant human IGF-II ovalbumin conjugate.

   Quantity: 20µl of serum

   Appearance: White Powder

   Specificity: The antiserum is specific for human IGF-II.
   Cross-reactivity with porcine, chicken and bovine IGF-II is 100 %
   and 85% respectively. Less than 0.1 % cross-reactivity is
   observed with ovine, insulin, human, rat and chicken IGF-I.

   Titre: At a dilution of 1/7500 the antiserum binds 25% of
   [125I]-labelled human IGF-I tracer (80Ci/g). Half-maximal inhibition
   (ED50) of radioligand binding is produced by 600pg of human
   IGF-II.

   Reconstitution: Dissolve in a suitable buffer solution appropriate
   for desired application.

   Storage/Stability: At least 2 years at 2-4°C (lyophilised). After
   reconstitution store at -20°C.

   Radioimmunoassay

   For radioimmunoassay (RIA), the recommended dilution of
   antiserum binds 25% of fresh [125I]-labelled human IGF-II tracer
   (80 Ci/g). Half-maximal inhibition (ED50) of radioligand binding is
   produced by 600 pg of human IGF-I and the limit of detection is
   approximately 20-30 pg.

   The relative potencies of the following peptides at inhibiting the
   binding of [125I]-labelled human IGF-II to this antiserum were found
   to be:
human IGF-II:      100%
human  IGF-I:     <0.1%
chicken IGF-II:    100%
chicken  IGF-I:  <0.1%
rat IGF-II: 100 %
rat IGF-I:              <0.1%
porcine IGF-II:    100%
bovine  IGF-II:      85%
bovine insulin:   <0.1%
 

 Datasheet for Anti-human IGF-II, polyclonal antiserum, rabbit