Product specifications monoclonal mouse anti-hepatitis B virus surface antigen (HBsAg):

Clones: HB5, HB6,  HB11, HB12, HB13, Hs33, Hs41.

Description: clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant HBsAg of ayw subtype (Clones HB5, HB6, HB11, HB13, HB12, Hs33, Hs41) and with native HBsAg (Clones NE3, NF5).

Specificity: Clones are reacting with the following HBsAg subtypes: ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-, ayw3 (Fer).

Isotypes: IgG1 for Clones NE3, HB11, HB13, HB12, HB6, HB5, Hs41 and B1.
IgG2a for Clones NF5, Hs33 and B5

Applications: ELISA.  Recommended pairs for sandwich immunoassay:
NE3 - NF5, HB12 - HB11, HB5 - HB6, HB13 - HB6, HB12 - HB6, Hs33 - Hs41,
Best pair: Hs33 - Hs41, sensitivity 50 pg/ml.

Purification: Clones B5 and B1 are purified by chromatography on protein A Sepharose. All other Clones are purified by chromatography on protein G Sepharose.

Formulation: PBS, pH 7.4, < 0.1 % sodium azide (NaN3).

Storage: + 4 °C.

 Datasheet for Anti-Hepatitis B Virus Surface antigen, monoclonal, mouse