Product specifications monoclonal mouse Anti-Glycoprotein 1 of Hantaan virus (HTNV). IgG fraction:

Clone: 1F5/G2.

Description: Mouse monoclonal antibody IgG fraction (clone 1F5/G2) obtained by immunizing mice with purified recombinant HTNV Glycoprotein 1 (G1). The IgG fraction was purified using Protein G-Sepharose.

Isotype: IgG1.

Storage: Store at -20ºC.

Stability: Stable at least one year at -20 ºC. Avoid repeated freezing and thawing.

Applications: This antibody can be used in ELISA (1:30,000 dilution) and Western blot (1:20,000 dilution) analysis. It recognizes specifically G1 glycoprotein of HTNV. Hantaviruses (family Bunyaviridae) are enveloped viruses that contain a tripartite, negative-stranded RNA genome encoding an RNA-dependent RNA polymerase (L), two integral membrane glycoproteins (G1 and G2), and a nucleocapsid (N) protein. The G1 and G2 glycoproteins form heterodimers and they attach the virion to a cell receptor and to promote fusion membrane after endocytosis of the virion. G1 can dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis. HTNV is a causative agent of severe hemorrhagic fever mainly in China and it is transmitted by the rodent Apodemus agrarius.

Formulation: Solution at 1.0 mg/ml in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.

References:

Yan, L. et al (2007) Emerg. Infect. Dis. 13, 1301-1306.
Löber, C. et al. (2001) Virology 289, 224-229.
Antic, D. et al. (1992) Virology 189, 324-328.
Yoo, D. & Kang, C.Y. (1987) Nucleic Acids Res. 15, 6299-6300.

 Datasheet for Anti-Hantavirus Hantaan strain (HTNV) glyprotein 1, monoclonal, mouse