Product Specifications Long TMEGF

The Product: Long TMEGF is an analog of humanepidermal growth    factor comprising the human EGF amino acid sequence plus a    53 amino acid N-terminal extension peptide. Long EGF has been developed as an inexpensive, high-quality potent analog of EGF for use as a growth factor supplement for serum-free or low serum cell culture. Long EGF is also suitable for animal growth studies and other general research applications where a low cost bulk reagent is required.   

Source: Produced recombinantly in E.coli using a patented expression system,  the active Long EGF molecule is correctly folded  and  purified by several chromatography steps.   

Purity: > 95% (as determined by N-terminal sequence analysis)   

Molecular Weight: 12298 daltons and a minor N-terminal formylated form of 12326 daltons - confirmed by Mass Spectrometry   

N-terminal sequence analysis of recombinant product: 5 residues >95% single sequence   

Biological Activity: ED50  <10 ng/ml   

Endotoxin (LAL): <0.25 EU/ug   

Stability: At least 2 years at 2-4°C (lyophilised)   

Appearance: White Powder   

State: Dried from 0.1 M acetic acid: under dry nitrogen @ slight    vacuum (-25kPa)   

Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl, but not 0.1M acetic acid), aliquot and store at -20°C.  For lower dilutions (e.g. <1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles.

This is a Novozymes GroPep specification. ibt is authorized distributor of GroPep. Long TMEGF is a traemark of GroPep.

 

Ref.:

1. The effect of benzo(a)pyrene on porcine urinary bladder epithelial cells analyzed for the expression of selected genes and cellular toxicological endpoints. Wolf et al. Toxicology, Volume 207, Issue 2, 14 February 2005, Pages 255-269.

2. Concomitant detection of CYP1A1 enzymatic activity and CYP1A1 protein in individual cells of a human urothelial cell line using a bilayer microfluidic device. Schumann et al. Analytical and Bioanalytical Chemistry (2008) 392:1159-1166.

Link to Abstract.

 Datasheet for LongTM EGF, recombinant protein