Product Specifications Des(2,3)[Ala31]IGF-I:

Des(2,3)[Ala31]IGF-I is a 68 amino acid analog of human IGF-I lacking Pro-Glu at positions 2-3 at the N-terminus and with a substitution of Ala for Tyr at position 31. Compared to [Ala31]IGF-I, Des(2,3)[Ala31]IGF-I has reduced binding to the Type 1 IGF receptor, but binding to acid-stripped human serum binding proteins (predominantly hIGFBP-3) is unaltered.

Source: Des(2,3)[Ala31]IGF-I is produced recombinantly as a fusion protein in E.coli using a patented expression system. The fusion protein containing Des(2,3)[Ala31]IGF-I is refolded, and after the leader peptide is removed, the Des(2,3)[Ala31]IGF-I product is purified to Receptor Grade using several chromatography steps.

Purity: > 95% (as determined by HPLC and N-terminal sequence analysis)

Molecular weight: Theoretical : 7330 daltons. Confirmed by Mass Spectrometry.

Appearance: Lyophilized white powder

N-terminal sequence analysis: 5 residues > 95% single sequence

Endotoxin (LAL): < 0.1 EU/µg

Biological Activity:

Type 1 IGF receptor binding assay - ED50 > 30 ng/ml

IGF binding protein assay - ED50 < 10 ng/ml

State:Dried from 0.1 M acetic acid and stored under dry nitrogen at a slight vacuum (-25kPa)

Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C or -80°C. For lower dilutions (e.g. < 1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles.

Storage/Stability:At least 2 years at 2-4°C (lyophilized)

Peptide can be biotinylated by ibt upon request.

This is a GroPep specification. ibt is authorized distributor of GroPep.

 Datasheet for Des(2,3)[Ala31]IGF-I, recombinant protein (Receptor Grade)