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Insulin-like Growth Factors (IGF´s), Biotinylated IGF´s and Analogs, Biotinylated Insulin
Human Des(1-3)-IGF-I, recombinant protein (Receptor Grade)
Des (1-3) Insulin-like Growth Factor-I, human, recombinant (Receptor Grade) Synonyms: Des(1-3)-IGF-1, Des (1-3) Insulin-like Growth Factor-1
Product Code DU
The Product: Des(1-3) insulin-like growth factor-I, [Des(1-3)IGF-I]
is a 67 amino acid analog of human IGF-I lacking the N-terminal
tripeptide Gly-Pro-Glu. Des(1-3) IGF-I is more potent than IGF-I in
vitro and in vivo. This increased potency is due to reduced
binding of Des(1-3)IGF-I to most of the IGF binding proteins
produced by cells. These binding proteins normally inhibit the
biological actions of IGF-I. The truncated peptide has been found in brain
and other tissues.
Source: Des(1-3)IGF-I is produced in E. coli using a
patented expression system. Several chromatography purification
steps are employed to yield the Receptor Grade product.
Purity: >95% (as determined by HPLC and N-terminal sequence
Molecular Weight: 7365 daltons - confirmed by Mass
N-terminal sequence analysis of recombinant product: 5
residues >95% single sequence
Biological Activity: (stimulation of protein synthesis in rat L6
myoblasts) ED50 <10 ng/ml
Endotoxin (LAL): <0.1 EU/ug
Stability: at least 2 years at 2-4Â°C (lyophilised)
Appearance: White Powder
State: Dried from 0.1 M acetic acid: under dry nitrogen at slight
Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M
acetic acid), aliquot and store at -20Â°C. For lower dilutions (e.g.
<1mg/ml) inclusion of a carrier protein is recommended. Avoid
This is a GroPep specification. ibt is authorized distributor of GroPep.
Datasheet for Human Des(1-3)-IGF-I, recombinant protein (Receptor Grade)