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Insulin-like Growth Factors (IGF´s), Biotinylated IGF´s and Analogs, Biotinylated Insulin
Chicken IGF-I, recombinant protein (Receptor Grade)
Insulin-like Growth Factor-I, Chicken, recombinant Synonyms: Igf-1, insulin-like growth factor-1, Somatomedin c, ILGF1, Mechano growth factor
Product Specifications Chicken IGF-I:
The Product: Chicken IGF-I is a polypeptide comprising 70 amino acids, eight of which differ from the amino acid sequence of human IGF-I. Chicken IGF-I has been shown to stimulate growth in many cell types, including rat L6 myoblasts and chicken embryo fibroblasts, hepatocytes,adipocytes and myoblasts.
Link to Sequence:
Source: Chicken IGF-I is produced recombinantly in E. coli using a patented expression system. The chicken IGF-I molecule is correctly folded and several chromatography purification steps are employed to yield Receptor Grade chicken IGF-I.
Purity: > 95% (as determined by HPLC and N-terminal sequence analysis)
Molecular Weight: 7738 daltons - confirmed by Mass Spectrometry
N-terminal sequence analysis of recombinant product: 5 residues >95% single sequence.
Biological Activity: (stimulation of protein synthesis in rat L6 myoblasts) ED50 <30 ng/ml
Endotoxin (LAL): <0.1 EU/ug
Storage/Stability: At least 2 years at 2-4Â° C (lyophilised)
Appearance: White Powder
State: Dried from 0.1 M acetic acid: under dry nitrogen @ slight vacuum (-25kPa)
Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20Â°C. For lower dilutions (e.g. <1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles.
Reference:Ongabesan et al:BMPs and BMPRs in chicken ovary and the effects of BMP-4 and â€“7 on granulosa cell proliferation and progesterone production in vitro. Am J Physiol Endocrinol Metab (July 29, 2003). 10.1152/ajpendo.00104.2003.
This is a GroPep specification. ibt is authorized distributor of GroPep.
Datasheet for Chicken IGF-I, recombinant protein (Receptor Grade)