Insulin-like Growth Factors (IGF´s), Biotinylated IGF´s and Analogs, Biotinylated Insulin

Bream IGF-I, recombinant protein (Receptor Grade)

Bream (Pagrus auratus) Insulin-like Growth Factor-I (Receptor Grade) Synonyms: Igf-1, insulin-like growth factor-1, Somatomedin c, ILGF1, Mechano growth factor

Product Specifications Bream IGF-I:

 

Recombinant red sea bream (Pagrus auratus) insulin-like growth factor-I s a 67 amino acid polypeptide. IGF-I stimulates growth and differentiation in many cell types and mediates many of the anabolic properties of growth hormone. Recombinant red sea bream (Pagrus auratus) IGF-I is identical to [Gly1] gilthead sea bream (Sparus aurata) IGF-I and [Gly1] black bream (Acanthopagrus butcheri) IGF-I, as glycine replaces the serine at position 1 in these proteins.

Link to Sequence:

Source: Red sea bream IGF-I is synthesized in E. coli using a patented expression system and the bream IGF-I is correctly folded and then purified by several liquid chromatography purification steps to yield receptor grade bream IGF-I.

Purity: > 95% (as determined by HPLC and N-terminal sequence analysis)

Molecular Weight:

7379 daltons – confirmed by Mass Spectrometry

Appearance: White Powder

N-terminal sequence analysis: 5 residues > 95% single sequence

Endotoxin (LAL): < 0.1 EU/µg

Biological Activity: (stimulation of protein synthesis in L6 myoblasts) ED50 < 5 nM

State: Lyophilised from 0.1 M acetic acid: under dry nitrogen at a slight vacuum (-25kPa)

Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilutions (e.g. < 1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles.

Storage/Stability: At least 2 years at 2-4°C (lyophilised)

This is a Gropep specification. ibt is authorized distributor of GroPep.

Rev. 2013-02

 Datasheet for Bream IGF-I, recombinant protein (Receptor Grade)

Category: Insulin-like Growth Factors (IGF´s), Biotinylated IGF´s and Analogs
Itemnumber: AGU020 AGU100
Price: Size: 
Price  EUR 230.00
Quantity: