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Antibodies to Insulin-like Growth Factor Binding Proteins (IGFBP´s)
Anti-human/rat IGFBP-3, polyclonal antibody, rabbit
Anti-human Insulin-like Growth Factor Binding Protein-3, polyclonal, rabbit
Product Code Specification:
The Product: The IGFBP-3 IgG fraction was purified from a polyclonal antiserum obtained from rabbits immunized with a synthetic peptide of unique sequence from the central domain of human IGFBP-3. The antibody has been characterized for immunohistochemistry and EIA.
Quantity: 20 ug. Specificity: In EIA the antibody does not cross-react with human IGFBP-1, -2, -4, -5 and â€“6.
Application: Characterized for the immunohistochemical detection of IGFBP-3 in OCT treated human liver sections and paraffin embedded sections of embryonic rat tissues. Titre: A final dilution of 1/500 for IHC in embryonic rat tissues with biotinylated IgG secondary antibody and detection with ExtrAvidin conjugated Cy3. A final dilution of 1/50 â€“ 1/100 for IHC in OCT treated human liver sections. Use at a final dilution of ~1/2,500 for EIA.
Reconstitution: Dissolve in 400 Âµl sterile water (1/20 dilution). Dilute further in a buffer solution appropriate for the desired application. Storage/Stability: In lyophilized form the antibody is stable for at least 2 years at 2 - 4 Â°C. After reconstitution, aliquot and store at -20 Â°C or -80 Â°C. Avoid repeated freezing and thawing.
This is a GroPep product specification. ibt is authorized distributor for GroPep products.
Datasheet for Anti-human/rat IGFBP-3, polyclonal antibody, rabbit